C. Elegans Lab Report

Genetics Lab Report We gathered tout tout ensemble of the materials unitedly which consisted of: a microscope, subaltern worm picks to pick-up the worms, petri-dishes of C. elegans worms, 2 blanched petri-dishes with no worms and a circle of worm food, ethanol, and a erupt. In the following experiments I hoped to learn what types of c. elegans herm mutations were dominant or recessive by crossing mutations and muster uping the F1 and F2 offspring. I in like manner insufficiency to learn how the breeding of these worms will affect the genotypes and phenotypes of the offspring. convention all the materials to the groups workspace, we took the petri dishes full of the worms and placed them contiguous to the microscope. The jump petri dish we placed nether the microscope at 37x was the 1 containing wild-type hermaphroditic offspring. The Next crosses that we experimented with were wild-type males with sma-9 hermaphrodites. Taking the worm-pick, and putting it in the f lame ensured that it was barren to use in the petri dish with the worms. Then, using that incision under the microscope, we picked out some worms that were in the L4 stage of outgrowth (shown in Diagram 1), both hermaphrodites and wild-type males and placed them in a altogether new petri dish to determine offspring.

They were quite small and were much(prenominal) a high number of them in the prepared petri dishes, more(prenominal) or less 500 in each. It was difficult to find the males in the dishes because they barely consisted of 5% or less of the population. The males were besides somewhat ruffianly to differentiate from the hermaphrodites. The difference is sho! wn to a cast down place in the realize below: In picture A, the picture shows a male worm, shown by the flat track. The epicene is in picture B where the tail is open and most wispy. It was extremely difficult to pick the worms up with the tools that we had in the prime(prenominal) lab. It seemed to just poke into the jelly-like material that the worms were on/in. Also, when picking up the worms it was often hard to tell how many...If you necessitate to get a full essay, order it on our website: BestEssayCheap.com

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